RTN4R Antibody

• Catalog Number: orb1247193
• Category: Antibodies
• Description: RTN4R Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-LQALPDDTFRDLGN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Experiments gave bands at approx 90-100kDa and 37+40+45kDa in Human brain lysate after 0.3ug/ml antibody staining. These bands were successfully blocked by the immunizing peptide and they correspond to earlier findings in the literature with different antibodies (Wang et al, J Neurosci. 2002 Jul 1;22(13):5505-15). This protein has a calculated MW of 50.7kDa according to NP_075380.1. Recommended concentration: 0.3-1ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: RTN4R
• UniProt ID: Q9BZR6
• NCBI: NP_075380.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RTN4R, Nogo-66 receptor, reticulon 4 receptor, HGN
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Experiments gave bands at approx 90-100kDa and 37+40+45kDa in Human brain lysate after 0.3ug/ml antibody staining. These bands were successfully blocked by the immunizing peptide and they correspond to earlier findings in the literature with different antibodies (Wang et al, J Neurosci. 2002 Jul 1;22(13):5505-15). This protein has a calculated MW of 50.7kDa according to NP_075380.1. Recommended concentration: 0.3-1ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247193 (0.3 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.