RRM2 Antibody [SAIC-30C-18], Rabbit IgG

• Catalog Number: orb1672054
• Category: Antibodies
• Description: RRM2 Antibody [SAIC-30C-18], Rabbit IgG
• Clonality: Recombinant
• Clone Number: SAIC-30C-18
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: The KLH-conjugated peptide "IEQEFLTEALPVK" derived from Ribonucleoside-diphosphate reductase subunit M2 (RRM2). RRM2 provides precursors required for DNA synthesis.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: RRM2
• UniProt ID: P31350
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: RR2, R2, RR2M, ribonucleotide reductase regulatory
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-RRM2 antibody SAIC-30C-18. Jurkat cell lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-30C-18 (orb1672054) at 1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow cytometry using the Anti-RRM2 antibody SAIC-30C-18. Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of SAIC-30C-18 (orb1672054 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-RRM2 (SAIC-30C-18). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-30C-18 (orb1672054) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672054- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.