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Catalog Number | orb1260463 |
---|---|
Category | Antibodies |
Description | RRM1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IP, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 593-792 of human RRM1 (NP_001024.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:20 - 1:50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 95kDa |
Target | RRM1 |
UniProt ID | P23921 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | RRM1, R1, RIR1, RR1 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:20 - 1:50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using RRM1 antibody (orb1260463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using RRM1 antibody (orb1260463) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 1 ug RRM1 antibody (orb1260463). Western blot was performed from the immunoprecipitate using RRM1 antibody (orb1260463) at a dilution of 1:500.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-Fr, IHC-P, IP, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human | |
Rabbit | |
Unconjugated |
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