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Catalog Number | orb393153 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to RPS6KA5 (Phospho-S376) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human |
Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S376 of human MSK1 protein. The exact sequence is proprietary. |
Dilution range | WB: 1:500:1000, IF/ICC: 1:100:500 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | RPS6KA5 |
Entrez | 9252 |
UniProt ID | O75582 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | MSK1; Ribosomal protein S6 kinase alpha-5; S6K-alp Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of MSK1 (Phospho-S376) expression in K562 (A), HEK293T (B) whole cell lysates. (Predicted band size: 89 kD; Observed band size: 90 kD)
Immunofluorescent analysis of MSK1 (Phospho-S376) staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IHC, WB | |
Human, Mouse, Rat | |
Unconjugated |
WB | |
Bovine, Canine, Human, Porcine, Rabbit, Rat |
ELISA, IF, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
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