RPS6 (phospho-S235) antibody

• Catalog Number: orb223999
• Category: Antibodies
• Description: Rabbit polyclonal antibody to RPS6 (Phospho-S235)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Primate, Rat, Sheep, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S235 of human RPS6 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IF/ICC: 1:100-1:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: RPS6
• Entrez: 20104, 6194, 29304, 100911372
• UniProt ID: P62753, P62754, P62755
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti 40S ribosomal protein S6 antibody, anti Phosp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RPS6 (Phospho-S235) expression in HeLa Calyculin A-treated (A), NIH3T3 (B) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 32 kD)

Immunofluorescent analysis of RPS6 (Phospho-S235) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).