RPS27A Rabbit Polyclonal Antibody

SKU: orb584328

Description

Rabbit polyclonal antibody to RPS27A

Research Area

Epigenetics & Chromatin, Molecular Biology

Images & Validation

• Tested Applications: WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Sheep, Yeast, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RPS27A
• Target: RPS27A
• Protein Sequence: Synthetic peptide located within the following region: LRGGAKKRKKKSYTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRECP
• Molecular Weight: 18 kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

UBC, S27A, CEP80, UBA80, HEL112, UBCEP1, UBCEP80

25 ug of the indicated Human whole cell extracts was loaded onto a 10-20% SDS-PAGE gel. 0.3 ug/ml of the antibody was used in this experiment. Protein is ubiquitinated which adds 8 kDa to the molecular weight.

Sample Tissue: Mouse Kidney, Antibody dilution: 1 ug/ml.

Positive control (+): 293T (2T), Negative control (-): Human Placenta (PL), Antibody concentration: 1 ug/ml.

WB Suggested Anti-RPS27A Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:1562500, Positive Control: 293T cell lysate. RPS27A is supported by BioGPS gene expression data to be expressed in HEK293T.

Quick Database Links

Gene Symbol

RPS27A

UniProt

P62979

RefSeq (Protein):NP_002945