RPL5 Antibody

• Catalog Number: orb1523906
• Category: Antibodies
• Description: RPL5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 247 and 297
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RPL5
• UniProt ID: P46777
• NCBI: NP_000960.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: 60S ribosomal protein L5; PPP1R135; protein phosph
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse RPL5 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPL5 antibody orb1523906 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human RPL5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPL5 antibody orb1523906 used for IP at 3 µg per reaction.

Detection of human RPL5 by immunohistochemistry.Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti-RPL5 (Cat. No. orb1523906) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human RPL5 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RPL5 (Cat. No. orb1523906) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB