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Catalog Number | orb2292254 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant RPA3. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1F4 |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human |
Isotype | IgG1 lambda |
Immunogen | RPA3 (AAH05264, 12 a.a. ~ 121 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | INAGMLAQFIDKPVCFVGRLEKIHPTGKMFILSDGEGKNGTIELMEPLDEEISGIVEVVGRVTAKATILCTSYVQFKEDSHPFDLGLYNEAVKIIHDFPQFYPLGIVQHD |
NCBI | AAH05264 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunoperoxidase of monoclonal antibody to RPA3 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]
RPA3 monoclonal antibody (M01), clone 1F4 Western Blot analysis of RPA3 expression in HL-60.
Western Blot analysis of RPA3 expression in transfected 293T cell line by RPA3 monoclonal antibody (M01), clone 1F4. Lane 1: RPA3 transfected lysate (13.6 KDa). Lane 2: Non-transfected lysate.
Western blot analysis of RPA3 over-expressed 293 cell line, cotransfected with RPA3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RPA3 monoclonal antibody (M01), clone 1F4 (Cat # orb2292254). GAPDH (36.1 kDa) used as specificity and loading control.
Western Blot detection against Immunogen (37.73 KDa).