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Catalog Number | orb1474308 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to RPA2 |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | IF, IP, WB |
Reactivity | Human |
Immunogen | Purified recombinant human RPA32/RPA2 protein fragments expressed in E.coli. |
Dilution range | WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) |
Form/Appearance | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
Conjugation | Unconjugated |
Target | RPA2 |
Entrez | 6118 |
UniProt ID | P15927 |
Source | Mouse |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
Alternative names | REPA2; RPA32; RPA34; Replication protein A 32 kDa Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of RPA2 expression in MCF7 (A), Hela (B), HUVEC (C) whole cell lysates. (Predicted band size: 29 kD; Observed band size: 32 kD)
Immunofluorescent analysis of RPA2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC, IP, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, IHC-P, WB | |
Bovine, Canine, Porcine, Sheep, Xenopus, Zebrafish | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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