Robo3.1 Antibody

• Catalog Number: orb1248151
• Category: Antibodies
• Description: Robo3.1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA
• Predicted Reactivity: Mouse
• Immunogen: The immunogen for this antibody is: C-PYGRPSFLSHGQGT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave bands at approx 100kDa and 80kDa in Mouse fetal Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 150kDa according to NP_001158239. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Robo3.1
• UniProt ID: Q9Z2I4
• NCBI: NP_001158239.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Robo3, roundabout homolog 3 (Drosophila), Rbig1, R
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave bands at approx 100kDa and 80kDa in Mouse fetal Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 150kDa according to NP_001158239. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Expiration Date: 12 months from date of receipt.