ROBO3 Antibody

• Catalog Number: orb1247933
• Category: Antibodies
• Description: ROBO3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC
• Predicted Reactivity: Canine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-RKDGARLKEEEGR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Western Blot: Preliminary experiments gave an approx 25kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 148kDa according to NP_071765.2. The 25kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Flow cytometry: This antibody was successfully used on rheumatoid arthritis synovial fibroblasts (RASF) as described in Denk et al, Arthritis Res Ther. 2010;12(2):R45.; PMID: 20298552.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ROBO3
• UniProt ID: Q96MS0
• NCBI: NP_071765.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ROBO3, roundabout, axon guidance receptor, homolog
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Western Blot: Preliminary experiments gave an approx 25kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 148kDa according to NP_071765.2. The 25kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Flow cytometry: This antibody was successfully used on rheumatoid arthritis synovial fibroblasts (RASF) as described in Denk et al, Arthritis Res Ther. 2010;12(2):R45.; PMID: 20298552.
• Expiration Date: 12 months from date of receipt.