Robo3 Antibody

• Catalog Number: orb1246999
• Category: Antibodies
• Description: Robo3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA
• Predicted Reactivity: Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-RRQKQRKELSHYT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 75-80kDa band in Mouse Brain and Rat Brain lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 143kDa according to NP_035378.1and AAD11628.1. The 75-80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Robo3
• UniProt ID: Q9Z2I4
• NCBI: AAD11628.1, NP_035378.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Robo3, roundabout homolog 3 (Drosophila), Rbig1, R
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 75-80kDa band in Mouse Brain and Rat Brain lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 143kDa according to NP_035378.1and AAD11628.1. The 75-80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Expiration Date: 12 months from date of receipt.