ROBO1 Antibody (aa1625-1650)

• Catalog Number: orb1539102
• Category: Antibodies
• Description: ROBO1 Antibody (aa1625-1650)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IHC-P, IP, WB
• Reactivity: Canine, Human, Mouse, Rat
• Isotype: IgG
• Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an C-Terminal region near amino acids 1625-1650 of Human ROBO-1.
• Concentration: 1.1 mg/ml
• Dilution range: ELISA (1:30000 - 1:60000), IF, IHC, IHC-P (2.5 µg/ml), IP, WB (1:500 - 1:3000)
• Conjugation: Unconjugated
• Target: ROBO1
• Storage: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide. 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
• Alternative names: ROBO1, Deleted in U twenty twenty, H-Robo-1, Round
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B81 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B81 was determined to be 2.5 ug/ml.
• Expiration Date: 12 months from date of receipt.