RNF138 Antibody

• Catalog Number: orb1525635
• Category: Antibodies
• Description: RNF138 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human, Mouse
• Immunogen: between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. A302-993A has not consistently performed satisfactorily when used for WB of RNF138 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RNF138.; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RNF138
• UniProt ID: Q8WVD3
• NCBI: NP_057355.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: NLK-associated RING finger protein; Nemo-like kina
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection of human RNF138 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-RNF138 antibody orb1525635 used for IP at 6 µg/mg lysate.

Detection of mouse RNF138 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-RNF138 (Cat. No. orb1525635) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human RNF138 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RNF138 (Cat. No. orb1525635) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB