RNASEH2A monoclonal antibody (M01), clone 3G5-F5

• Catalog Number: orb2291545
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant RNASEH2A.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3G5-F5
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: RNASEH2A (AAH11748, 1 a.a. ~ 299 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MDLSELERDNTGRCRLSSPVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERERLFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQVFVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEKLQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWEDSASENQEGLRKITSYFLNEGSQARPRSSHRYFLERGLESATSL
• NCBI: AAH11748
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged RNASEH2A is 1 ng/ml as a capture antibody.

RNASEH2A monoclonal antibody (M01), clone 3G5-F5 Western Blot analysis of RNASEH2A expression in Jurkat.

Western Blot analysis of RNASEH2A expression in transfected 293T cell line by RNASEH2A monoclonal antibody (M01), clone 3G5-F5. Lane 1: RNASEH2A transfected lysate(33.4 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of RNASEH2A over-expressed 293 cell line, cotransfected with RNASEH2A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RNASEH2A monoclonal antibody (M01), clone 3G5-F5 (Cat # orb2291545). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (58.63 KDa).