RNASEH2A Antibody

• Catalog Number: orb1523528
• Category: Antibodies
• Description: RNASEH2A is the catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. RNASEH2A participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Isotype: Whole IgG
• Immunogen: Between 250-300aa
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Liquid
• Purity: Antigen Affinity Purified
• Conjugation: Unconjugated
• Target: RNASEH2A
• UniProt ID: O75792
• NCBI: NP_006388.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: AGS4; aicardi-Goutieres syndrome 4 protein; JUNB;
• Note: For research use only
• Application notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight. Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody. Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate with 5% Normal Pig Serum added to the blocking buffer.
• Expiration Date: 12 months from date of receipt.

Detection of human RNASEH2A by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HeLa, and 293T cells. Antibodies: Affinity purified rabbit anti-RNASEH2A antibody orb1523528 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human RNASEH2A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-RNASEH2A antibody orb1523528 used for IP at 6 µg/mg lysate.