RLTPR Antibody (APC)

• Catalog Number: orb1821024
• Category: Antibodies
• Description: RLTPR / CARMIL2 (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; capping protein regulator and myosin 1 linker 2), also known as LRRC16C, is a cytosolic protein, which with high affinity binds CAPZA2 (capping protein muscle actin Z-line alpha 2) and decreases CAPZA2 affinity for actin barbed ends. RLTPR / CARMIL2 increases the rate of actin filament elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Its interaction with CAPZA2 is essential for lamellipodial protrusion and cell translocation. RLTPR / CARMIL2 is crutial for T cell costimulation via CD28 and this property seems to be independent on its actin-uncapping function. The lack of functional RLTPR / CARMIL2 molecules impeded the differentiation toward Th1 and Th17 fates of both human and murine CD4+ T cells and leads to combined immunodeficiency. Expression of RLTPR / CARMIL2 was also detected in human and murine B cells, but it seems not to be involved in BCR-mediated signaling.
• Clonality: Monoclonal
• Clone Number: EM-53
• Tested applications: FC
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1 kappa
• Immunogen: Murine RLTPR
• Concentration: 0.1 mg/ml
• Conjugation: APC
• Target: RLTPR
• Entrez: 146206
• UniProt ID: Q6F5E8
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: CARMIL2, LRRC16C
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of cells stained using anti-RLTPR (EM-53) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 5 μg/ml, same as RLTPR APC antibody concentration, black-dashed) in flow cytometry analysis (intracellular staining) of RLTPR transfected BI-141 cells.