RIPK2 Antibody

• Catalog Number: orb1239926
• Category: Antibodies
• Description: RIPK2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: RICK antibody was raised against a peptide corresponding to 20 amino acids near the amino terminus of human RICK.The immunogen is located within the first 50 amino acids of RICK.
• Concentration: 1 mg/ml
• Dilution range: RICK antibody can be used for detection of RICK by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 59 kDa Observed: 59 kDa
• Target: RIPK2
• UniProt ID: O43353
• NCBI: O43353
• Storage: RICK antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: RICK Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: RICK Antibody: CCK, RICK, RIP2, CARD3, GIG30, CARD
• Note: For research use only
• Application notes: RICK antibody can be used for detection of RICK by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RICK in (A) HeLa, (B) Ramos and (C) EL4 cell lysate with RICK antibody at 1 µg/mL.

Immunocytochemistry of RICK in A431 cells with RICK antibody at 10 µg/mL.

Immunofluorescence of RICK in K562 cells with RICK antibody at 20 µg/ml.

Immunofluorescence of RICK in K562 cells with RICK antibody at 20 µg/mL. Red: RICK Antibody (orb1239926) Blue: DAPI staining.

Immunocytochemistry of RICK in K562 cells with RICK antibody at 2.5 µg/mL.