RIOK3 monoclonal antibody (M01), clone 4A12-1D2

• Catalog Number: orb2291830
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant RIOK3.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4A12-1D2
• Tested applications: ELISA, IP, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: RIOK3 (AAH39729, 1 a.a. ~ 516 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGENIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDDPYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKNTARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHAYSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE
• NCBI: AAH39729
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged RIOK3 is approximately 0.3 ng/ml as a capture antibody.

Immunoprecipitation of RIOK3 transfected lysate using anti-RIOK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RIOK3 MaxPab rabbit polyclonal antibody.

RIOK3 monoclonal antibody (M01), clone 4A12-1D2. Western Blot analysis of RIOK3 expression in MCF-7.

Western Blot analysis of RIOK3 expression in transfected 293T cell line by RIOK3 monoclonal antibody (M01), clone 4A12-1D2. Lane 1: RIOK3 transfected lysate (59.1 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (82.5 KDa).