RIF1 Antibody

• Catalog Number: orb1248675
• Category: Antibodies
• Description: RIF1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-RNLQSRWRSPSHEN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Immunofluorescence: Expression of the protein seen in the nucleus of NIH3T3 and U2OS cells. Recommended concentration: 10ug/ml
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 270 kDa
• Target: RIF1
• UniProt ID: Q5UIP0
• NCBI: NP_001171134.1, NP_060621.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RIF1, RAP1 interacting factor homolog (yeast), DKF
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Immunofluorescence: Expression of the protein seen in the nucleus of NIH3T3 and U2OS cells. Recommended concentration: 10ug/ml
• Expiration Date: 12 months from date of receipt.

orb1248675 (1 ug/ml) staining of HeLa and Mouse D3 (ES) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248675 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining. The nuclear stain is DAPI.

orb1248675 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue).