Ribonuclease Inhibitor/RNH1 Antibody

• Catalog Number: orb334618
• Category: Antibodies
• Description: Ribonuclease Inhibitor/RNH1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human RNH1 (2-37aa SLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDD), different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 49973 MW
• UniProt ID: P13489
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Ribonuclease inhibitor;Placental ribonuclease inhi
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A549 cells using anti-RNH1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A549 cells using anti-RNH1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A431 cells using anti-RNH1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of RNH1 using anti-RNH1 antibody.Lane 1:human placenta tissue;2:human HeLa cell;3:human SK-OV-3 cell;4:human Jurkat cell;5:human U-87MG cell;6:monkey COS-7 cell;7:human SW620 cell;8:human Caco-2 cell.

WB analysis of RNH1 using anti-RNH1 antibody.Lane 1:rat brain tissue;2:rat testis tissue;3:rat lung tissue;4:mouse brain tissue;5:mouse testis tissue;6:mouse lung tissue;7:mouse HEPA1-6 cell.

IHC analysis of RNH1 using anti-RNH1 antibody.RNH1 was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of RNH1 using anti-RNH1 antibody.RNH1 was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of RNH1 using anti-RNH1 antibody.RNH1 was detected in paraffin-embedded section of human lung cancer tissues.

IHC analysis of RNH1 using anti-RNH1 antibody.RNH1 was detected in paraffin-embedded section of human lung cancer tissues.