Ribonuclease A Antibody

• Catalog Number: orb750605
• Category: Antibodies
• Description: RNASE1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Bovine
• Isotype: Antiserum
• Immunogen: Anti-Ribonuclease A was produced by repeated immunizations with bovine pancreatic Ribonuclease A.
• Antibody Type: Primary Antibody
• Concentration: 90 mg/mL
• Dilution range: ELISA: 1:30,000 - 1:150,000, IP: 1:100, WB: 1:3,000 - 1:15,000
• Form/Appearance: Lyophilized
• Purity: Anti-Ribonuclease A was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Ribonuclease A [Bovine Pancreas]. Cross reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: P61823
• NCBI: AAI49530.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: None
• Alternative names: rabbit anti-Ribonuclease A Antibody, Ribonuclease
• Note: For research use only
• Application notes: Anti-Ribonuclease A Antibody is suitable for western blotting, IP and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Rab27A deficiency caused increased expression of Rab27B, but did not affect its activity.(A and B) Examples of total lysates of isolated pancreatic acinar cells from wild-type C3H/HeSnJ or ashen mice were analyzed by western blot. Each lane represents samples from one mouse. (B) Densitometry analysis on the western blot results from all samples run as in (A). The results are mean ± SE from five mice of each genotype. *P < 0.05. (C) Active form of Rab27B and Rab3D at basal level in isolated acini was examined by GST-SHD and GST-Rim pulldown, respectively. Pulldown fractions were analyzed by western blot. This experiment was repeated three times with similar results. (D) The expression of major digestive enzymes (amylase, chymotrypsin, lipase, and elastase) and other Rab proteins (Rab6 and Rab11) was also not changed in western blots on lysates from isolated ashen mouse acinar cells. (E) The expression of major digestive enzymes (amylase, chymotrypsin, elastase, carboxypeptidase A and ribonuclease A) was also not changed in western blots of purified ashen mouse zymogen granules.