Ribonuclease 3/ECP Rabbit Polyclonal Antibody

• Catalog Number: orb500883
• Category: Antibodies
• Description: Ribonuclease 3/ECP Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, WB
• Predicted Reactivity: Human
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Ribonuclease 3 (45-160/160aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ICC/IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 16 kDa
• Target: RNASE3
• UniProt ID: P12724
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: eosinophil cationic protein; EAR; Ear11; Ear4; EAR
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

RNASE3 Antibody (C-term) (orb500883) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the RNASE3 antibody detected the RNASE3 protein (arrow).

Western blot analysis of lysate from human spleen tissue lysate, using RNASE3 Antibody (C-term) (Cat. #orb500883), diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35 ug.

Confocal immunofluorescent analysis of RNASE3 Antibody (C-term) (orb500883) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).