Rhodamine Antibody

SKU: orb344402

Description

Rhodamine antibody

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:30,000, IHC: 1:1,000 - 1:10,000, WB: 1:1,000 - 1:10,000
• Application Notes:
  This protein A purified monoclonal antibody against rhodamine reacts with most derivative molecules and has been tested for use in ELISA. Optimal concentration in other immunoassays should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 11H10
• Immunogen: Anti-RHODAMINE Monoclonal Antibody was produced after repeated immunizations of balb/c mice with rhodamine conjugated KLH.
• Purity: RHODAMINE Monoclonal Antibody was protein A purified and reacts specifically with Rhodamine and its derivatives. Rhodamine isomer 5 and isomer 6 are reactive as TAMRA, as well as TRITC conjugated proteins. No reaction is observed against Texas Red.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.115 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Mouse Anti-Rhodamine Antibody

Dot Blot of Mouse anti-Rhodamine Monoclonal Antibody. Antigen: Row 1 - Rhodamine Conjugated Streptavidin. Row 2 - Streptavidin. Load: Column 1 - 100 ng. Column 2 - 33.3 ng. Column 3 - 11.1 ng. Column 4 - 3.70 ng. Column 5 - 1.23 ng. Primary antibody: Mouse anti-Rhodamine at 1:1000 for 60 min at RT. Secondary antibody: HRP mouse secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 1 HR at RT.

Rhodamine core structure. TRITC (tetramethylrhodamine-5-(and-6)-isothiocyanate) is the base tetramethylrhodamine molecule functionalized with an isothiocyanate reactive group (–N = C = S) at one of two hydrogen atoms on the bottom ring of the structure. This derivative is reactive towards primary amine groups on proteins, peptides and other biomolecules. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.