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RGR Antibody (Center)
Catalog Number: orb1927659
| Catalog Number | orb1927659 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB24687 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 31874 Da |
| Target | This RGR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-291 amino acids from the Central region of human RGR. |
| UniProt ID | P47804 |
| NCBI | NP_002912.2, NP_001012738.1, NP_001012740.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | RPE-retinal G protein-coupled receptor, RGR Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
RGR Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes: Anti-RGR Antibody (Center) at 1:1000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: PANC-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
RGR Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RGR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
