RFP antibody (Biotin)

• Catalog Number: orb345914
• Category: Antibodies
• Description: RFP antibody (Biotin)
• Species/Host: Gallus
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Other
• Isotype: IgY
• Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000, IF: 1:100, WB: 1:1,000 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot and immunoelectrophoresis against anti-biotin, anti-chicken serum, and RFP.
• Conjugation: Biotin
• UniProt ID: Q9U6Y8
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: chicken anti-RFP antibody biotin conjugation, biot
• Note: For research use only
• Application notes: Anti-RFP is designed to detect recombinant RFP. This antibody has been tested to detect RFP by immunoblotting and immunofluorescence and is suitable for use in immunohistochemistry. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of nitrocellulose membrane using RFP antibody (Biotin)