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Revitablot Western Blot Stripping Buffer

Catalog Number: orb348648

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb348648
CategoryTools
DescriptionRevitablot Western Blot Stripping Buffer
Tested applicationsWB
Concentration< p>1X

Dilution rangeWB: Use undiluted.
Form/AppearanceLiquid
PurityRevitablot Western Blot Stripping Buffer contains solutions in a proprietary combination to enhance the removal of bound antibodies from western blot membranes for repeated use. The proprietary formulation of the solution ensures high stripping efficiency with low backgrounds.
ConjugationUnconjugated
Hazard InformationNon-Toxic
StorageStore container at room temperature (18° to 26° C) prior to opening.
Buffer/PreservativesPreservative: None. Stabilizer: None
Alternative namesStripping solution, Western Blot stripping buffer,
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NoteFor research use only
Application notesRevitablot Western Blot Stripping Buffer is ready-to-use as a working 1X solution and requires no further dilution.
Expiration Date12 months from date of receipt.
Revitablot Western Blot Stripping Buffer

Western Blot comparison of Revitablot™ Western Blot Stripping Buffer using Fluorescent labeled Primary Antibodies. Lane 1: 7.5 µg HCT-116 cell lysates. Lane 2: 22.5 µg HCT-116 cell lysates. Lane 3: 15.0 µg HCT-116 cell lysates. Block: 5% BLOTTO for 1 hr at room temperature. Blot A first probed with: Primary antibody: Mouse anti-AKT pS473 at a dilution of 1:1000 overnight at 4°C. Imaging: 650nm. Membranes were then incubated with Revitablot™ 5 minutes at room temperature, followed by re-blocking with 5% BLOTTO for 2 hours at room temperature. Blot B (Blot A reprobed) with: Primary antibody: Rabbit anti-beta actin DyLight 488 Conjugated at a dilution of 1:1000 for 4 hours at room temperature. Imaging: 488nm and 650nm.

Revitablot Western Blot Stripping Buffer

Western Blot comparison of Revitablot™ Western Blot Stripping Buffer. Lane 1: 70 µg HeLa whole cell lysates. Lane 2: 10 µg HeLa whole cell lysates. Blot A first probed with: Primary antibody: Rabbit Anti-STAT5 (p/n orb345603) at a dilution of 1:1000 overnight at 4°C. Secondary antibody: HRP Goat anti-rabbit (p/n orb347654) at 1:10000 dilution using FemtoMax Super Sensitive Chemiluminescent HRP Substrate. Membranes were then incubated with Revitablot and a competitor's product for 20 minutes at room temperature, followed by re-blocking with 5% BLOTTO for 2 hours at room temperature. Blot B (Blot A reprobed) with: Primary antibody: Rabbit anti-tubulin (p/n orb345510) at a dilution of 1:1000 overnight at 4°C. Secondary antibody: HRP Goat anti-rabbit (p/n orb347654) at 1:10000 dilution using FemtoMax Super Sensitive Chemiluminescent HRP Substrate.