Retnla Antibody

• Catalog Number: orb1272748
• Category: Antibodies
• Description: Retnla Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mRELMα. Murine RELMα specific antibody was purified by affinity chromatography employing immobilized mRELMα matrix.
• Concentration: batch dependent
• Dilution range: ELISA:Indirect:To detect mRELMa by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMa.SandwichTo detect mRELMa by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine RELMa as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMa. Western Blot:To detect mRELMa by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRELMa is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: Retnla
• UniProt ID: Q9EP95
• NCBI: Q9EP95
• Storage: RELMa antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: HIMF, Xcp2, Fizz1, RELMa, Fizz-1, RELMalpha, RELM-
• Note: For research use only
• Application notes: ELISA:Indirect:To detect mRELMa by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMa.SandwichTo detect mRELMa by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine RELMa as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMa. Western Blot:To detect mRELMa by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRELMa is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect Murine RELM-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELM-alpha (orb1272747) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine RELM-alpha.

To detect Murine RELM-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELM-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Murine RELM-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELM-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained 4% PFA, paraffin-embedded sections of murine cecum tissue (with positive cells infiltrating the Lamina Propia of a Trichuris muris infected mouse). The primary antibody was incubated at 4 ng/mL overnight at 4 °C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM Citric Acid was used. Optimal concentrations and conditions may vary.