Retinoid X Receptor alpha/RXRA Antibody

• Catalog Number: orb402244
• Category: Antibodies
• Description: Retinoid X Receptor alpha/RXRA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human RXRA recombinant protein (Position: A226-T462).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 51-55 kDa
• UniProt ID: P19793
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Retinoic acid receptor RXR-alpha; Nuclear receptor
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-RXRA antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of RXRA using anti-RXRA antibody.Lane 1:human HeLa cell;2:human COLO-320 cell;3:human A431 cell;4:human MCF-7 cell;5:rat heart tissue;6:mouse heart tissue.

IF analysis of RXRA using anti-RXRA antibody. RXRA was detected in immunocytochemical section of A431 cells.

IHC analysis of RXRA using anti-RXRA antibody.RXRA was detected in paraffin-embedded section of mouse liver tissue.

IHC analysis of RXRA using anti-RXRA antibody.RXRA was detected in paraffin-embedded section of rat liver tissue.