RET Antibody (Ascites)

• Catalog Number: orb652063
• Category: Antibodies
• Description: Mouse monoclonal antibody to RET
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 188CT11.2.3
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgM,K
• Immunogen: Recombinant Protein
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-16000
• Form/Appearance: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
• Conjugation: Unconjugated
• MW: 124319
• Target: RET
• UniProt ID: P07949
• NCBI: NP_065681.1, NP_066124.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-Proto-oncogene tyrosine-protein kinase recept
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-RET Antibody (Ascites) at 1:1000 dilution + mouse heart lysate. Secondary Goat Anti-mouse IgM, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 124319 Da. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of RET Antibody (Ascites) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

RET Monoclonal (Ascites) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RET Monoclonal (Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.

RET western blot analysis in A549 cell line lysates (35 μg/lane). This demonstrates the RET antibody detected the RET protein (arrow).