RepSox

SKU: orb1225030

Description

RepSox (E-616452, SJN-2511) is a potent, selective inhibitor of TGF-β type I receptor ALK5 with Ki of 25 nM, inhibits ALK5 autophosphorylation with IC50 of 4 nM, can replace the central reprogramming factor Sox2 by inducing nanog; specifically acts by inhibiting Tgf-β signaling, does not act by inducing Sox2 expression in the target fibroblasts; efficiently replaces transgenic Sox2 in the absence of VPA and c-Myc, as well as in both embryonic and adult fibroblasts; demonstrates effectivity at generating iPSCs.

Key Properties

• CAS Number: 446859-33-2
• MW: 287.3186
• Purity: >98% (HPLC)
• Formula: C₁₇H₁₃N₅
• SMILES: CC1=CC=CC(C2=NNC=C2C3=NC4=CC=CN=C4C=C3)=N1
• Target: TGFBR
• Solubility: DMSO: ≥ 52 mg/mL

Bioactivity

• In Vitro:
  RepSox (GMP) (10 μM) induces MEFs differentiating to chemically induced pluripotent stem cells (CiPSCs). RepSox (GMP) (10 μM, in stage I-Stage III induction medium) induces hCiPS cells from HEFs. RepSox (GMP) together with Forskolin (HY-15371) increases the number of proliferative cells in MEFs expressing MyoD, confirmed by increased EdU incorporation.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E-616452 | SJN 2511 | E616452 | SJN2511 | E 616452 | SJN-2511

Quick Database Links

Gene Symbol

TGFBR