Repo-Man rabbit pAb

• Catalog Number: orb766215
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Repo-Man
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human CDCA2. AA range:511-560
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 113
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-CDCA2 antibody, anti-Cell division cycle-asso
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Repo-Man Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Western blot analysis of lysates from 293 cells, using CDCA2 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).