Reparixin

SKU: orb1303100

Description

Reparixin

Research Area

Cell Biology, Immunology & Inflammation, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 266359-83-5
• MW: 283.39
• Purity: 98.00%
• Formula: C₁₄H₂₁NO₃S
• SMILES: CC(C)Cc1ccc(cc1)[C@@H](C)C(=O)NS(C)(=O)=O
• Target: CXCR
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.06 mM);DMSO:50 mg/mL (176.44 mM)

Bioactivity

• Target IC50:
  CXCR1:1 nM|CXCR2:100 nM|CXCR1 (Ile43Val):80 nM (in L1. Cells)|CXCR1 (wild-type):5.6 nM (in L1. Cells)
• Cell Research:
  L1.2 Cell suspension (1.5- × 06 cells/mL) is incubated at 37℃or 15 min in the presence of Vehicle or of Reparixin (1 nM-1 μM) and Next seeded in triplicates in the upper compartment o the chemotactic chamber. Different agonists are seeded in the lower compartment o the chamber a the following concentration : 1 nM CXCL8, 0.03 nM fMLP, 10 nM CXCL1, 2.5 nM CCL2, 30 nM C5a the chemotactic chamber is incubated at 37℃n air with 5% CO2 for 45 min (human PMNs) or 2 h (monocytes). A the end of incubation the filter is removed, fixed, and stained and five oil immersion fields at high magnification (10 × are counted for Each migration well after sample coding. L1.2 migration is evaluated using 5 μM pore size Transwell filters.
• Animal Research:
  C57BL/6J mice (8-10 weeks old/20-25 g) are used the subcutaneous administration of Reparixin (30 mg/kg) is performed 60 minutes before cerebral ischemia induction the animals are divided int the following three Experimental groups: Sham (i.e. the group in whic the arteries are visualized, but there is no occlusion o the middle cerebral artery), Vehicle (i.e. the group pre-treated wit the Vehicle, phosphate buffer solution, 60 minutes before MCAo) and Reparixin (i.e. the group pre-treated wit the drug 60 minutes before MCAo). To evaluate neurological signs secondary to MCAo the animals are assessed wit the SHIRPA battery 24 h after reperfusion.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

DF 1681Y, CXCR2, CXCR1, CXC chemokine receptors, CXCR, inhibit, Inhibitor, Repertaxin, Reparixin

Compound Identifiers

PubChem CID

9838712