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Catalog Number | orb669118 |
---|---|
Category | Antibodies |
Description | REN/Renin Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence of human REN/Renin (RKFYTEFDRRNNRIGFALAR). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
UniProt ID | P00797 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
IHC analysis of REN/Renin using anti-REN/Renin antibody (orb669118). REN/Renin was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-REN/Renin Antibody (orb669118) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.
ELISA, IHC, IHC-P, WB | |
Canine, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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