RelA, Phospho (S536) Antibody

• Catalog Number: orb1527301
• Category: Antibodies
• Description: RelA, Phospho (S536) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Immunogen: surrounding serine 536
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:200 - 1:1,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RelA, Phospho (S536)
• UniProt ID: Q04206
• NCBI: NP_068810.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: p65; NF-kappa-B transcription factor p65; v-rel re
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human phospho RelA-S536 by western blot. Samples: Whole cell lysate (15 and 50 µg) from Jurkat cells treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RelA (S536) antibody orb1527301 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Western blot for total RelA using affinity purified rabbit anti-RelA antibody at 0.1 µg/ml with exposure time of 75 seconds.

Detection of human Phospho RelA (S536) by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho RelA (S536) (Cat. No. orb1527301) used at a dilution of 1: 200 (1µg/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).