RelA, Phospho (S529) Antibody

• Catalog Number: orb1527299
• Category: Antibodies
• Description: RelA, Phospho (S529) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Immunogen: surrounding serine 529
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RelA, Phospho (S529)
• UniProt ID: Q04206
• NCBI: NP_068810.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: p65; NF-kappa-B transcription factor p65; v-rel re
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Phospho RelA (S529) by western blot. Samples: Whole cell lysate (50 µg) from Jurkat cells that were treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho RelA (S529) antibody orb1527299 used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Phospho RelA (S529) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that were treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibodies: RelA was immunoprecipitated with affinity purified rabbit anti-RelA antibody. Immunoprecipitated RelA was treated with phosphatase or mock treated. Affinity purified rabbit anti-Phospho RelA (S529) antibody orb1527299 used for WB at 1 µg/ml.