RelA, Phospho (S468) Antibody

• Catalog Number: orb1527297
• Category: Antibodies
• Description: RelA, Phospho (S468) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Immunogen: surrounding serine 468
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RelA, Phospho (S468)
• UniProt ID: Q04206
• NCBI: NP_068810.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: p65; NF-kappa-B transcription factor p65; v-rel re
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Phospho RelA (S468) by western blot. Samples: Whole cell lysate (50 µg) from Jurkat cells treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RelA (S468) antibody orb1527297 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of mouse Phospho RelA (S468) by immunohistochemistry. Samples: FFPE sections of mouse renal cell carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho RelA (S468) (Cat. No. orb1527297) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human Phospho RelA (S468) by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho RelA (S468) (Cat. No. orb1527297) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).