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Item 1 of 3
REG3A Antibody (N-term)
Catalog Number: orb1928041
| Catalog Number | orb1928041 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB21532 |
| Tested applications | FC, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 19395 Da |
| Target | This REG3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human REG3A. |
| UniProt ID | Q06141 |
| NCBI | NP_620355.1, NP_620354.1, NP_002571.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Regenerating islet-derived protein 3-alpha, REG-3- Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
REG3A Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from RPMI-8226 cell line, using REG3A Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug per lane.
All lanes: Anti-REG3A Antibody (N-term) at 1:1000 dilution. Lane 1: Human pancreas tissue lysate. Lane 2: PANC-1 whole cell lysate. Lane 3: U-2OS whole cell lysate. Lane 4: A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
