REDD1 Antibody

• Catalog Number: orb1527090
• Category: Antibodies
• Description: REDD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IP, WB
• Reactivity: Human
• Immunogen: between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:4,000; IP - 5 - 15 µg/mg lysate; ICC-IF - 1:500 - 1:2,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: REDD1
• UniProt ID: Q9NX09
• NCBI: NP_061931.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: protein regulated in development and DNA damage re
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human REDD1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Lysate was prepared from cells that had been treated with cobalt chloride (A and B) or mock treated (A). Antibodies: Affinity purified rabbit anti-REDD1 antibody orb1527090 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. REDD1 was also immunoprecipitated, albeit poorly, by rabbit anti-REDD1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Detection of human REDD1 by immunocytochemistry.Sample: NBF-fixed asynchronous, Cobalt-treated HeLa cells. Antibody: Affinity purified rabbit anti-REDD1 (Cat. No. orb1527090) used at a dilution of 1: 500 (2 µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG H&L cross-adsorbed Antibody DyLight®594 used at a dilution of 1: 100.