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Catalog Number | orb1526471 |
---|---|
Category | Antibodies |
Description | RECQ5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | between 650 and 700 |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 5 - 15 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | RECQ5 |
UniProt ID | O94762 |
NCBI | NP_004250.4 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | RecQ protein-like 5; RECQ5; DNA helicase, RecQ-lik Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human RECQ5 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RECQ5 antibody orb1526471 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. RECQ5 was also immunoprecipitated by rabbit anti-RECQ5 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
WB | |
Animal, Canine, Guinea pig, Human, Mouse, Rat | |
Canine, Equine, Guinea pig, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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