Recombinant VEGF (Vascular Endothelial Growth Factor) Antibody

• Catalog Number: orb2312720
• Category: Antibodies
• Description: Recombinant VEGF (Vascular Endothelial Growth Factor) Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: VEGFA/7757R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Recombinant fragment (around aa1-200) of human VEGFA protein (exact sequence is proprietary)
• Antibody Type: Recombinant Antibody
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 19-22kDa (reducing) and 38kDa-44kDa (non-reducing)
• Target: VEGFA
• Entrez: 7422
• UniProt ID: P15692
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MVCD1; Vascular Endothelial Growth Factor A (VEGF-
• Note: For research use only
• Application notes: Positive Control: Tumor cells in astrocytomas, breast or ovarian carcinomas.
• Expiration Date: 12 months from date of receipt.