Recombinant TNF-α, Mouse (P. pastoris-expressed)

• Catalog Number: orb1494607
• Category: Proteins
• Description: Sharing 79% sequence identity with human Tumor Necrosis Factor-alpha (hTNF-α), mouse Tumor Necrosis Factor-alpha (mTNF-α) is a cytokine mainly expressed by immune cells [1]. A type II transmembrane protein, TNF-α is further proteolytically processed to a soluble form [2]. The trimeric active TNF-α[3] then exerts its diverse biological properties including gapoptosis, inflammation, autoimmunity and cell proliferation by binding to TNF Receptor 1 and 2 [4]. Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) produced in P.pastoris is a fully biologically active polypeptide chain of 152 amino acids with a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 17kDa, observed by reducing SDS-PAGE.
• Protein Sequence: SQNSSD KPVAHVVANH QVEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLVYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV NLLSAVKSPC PKDTPEGAEL KPWYEPIYLG GVFQLEKGDQ LSAEVNLPKY LDFAESGQVY FGVIAL
• Source: P. pastoris
• Biological Activity: ED501 x 10ˆ8units/mg.
• Endotoxins: < 1 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmTNF-αshould be stable up to 1 week at 4°C or up to 2 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: TNF-alpha, TNF-α; Tumornecrosis factor ligand supe
• Note: For research use only
• Application notes: Reconstituted in ddH2O or PBS at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.