Recombinant M-CSF, Mouse

• Catalog Number: orb1494797
• Category: Proteins
• Description: Macrophage Colony Stimulating Factor (M-CSF) ,also known as CSF1,is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells.M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity.M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation.Recombinant mouse Macrophage Colony Stimulating Factor (M-CSF) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 156 amino acids each. A fully biologically active molecule, rmM-CSF has a molecular mass of 30 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 30 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: MKEVSEHCS HMIGNGHLKV LQQLIDSQME TSCQIAFEFV DQEQLDDPVC YLKKAFFLVQ DIIDETMRFK DNTPNANATE RLQELSNNLN SCFTKDYEEQ NKACVRTFHE TPLQLLEKIK NFFNETKNLL EKDWNIFTKN CNNSFAKCSS RDVVTKP
• Source: Escherichia coli.
• Biological Activity: ED50 3.3×10ˆ5 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant Mouse Macrophage Colony Stimulating Factor(M-CSF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse M-CSF should be stable up to 1 week at 4°C or up to 2 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against 50mMTris,150mMNaCl, pH 8.0.
• Alternative names: Macrophage Colony Stimulating Factor, CSF-1, Lanim
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/ml.
• Expiration Date: 6 months from date of receipt.