Recombinant IL-8/CXCL8 (8-79aa), Human

• Catalog Number: orb1494812
• Category: Proteins
• Description: Interleukin-8 is one of the first discovered chemokines, and belongs to the CXCL family, in which the first two conserved cysteines are separated by one residue. In vivo, IL-8 exists in two forms: 77 a.a. produced by endothelial cells, and the more active 72 a.a. produced by monocytes. The receptors of IL-8 are the seven-helical G-protein coupled receptors CXCR1 and CXCR2, exclusively expressed on neutrophils. The functions of IL-8 are to induce rapid changes in cellular shape, activate the integrins, and release the granule contents of neutrophils. Thus, IL-8 can enhance the antimicrobial actions of defense cells.Recombinant human I Interleukin-8/CXCL8 (rhIL-8) produced in E.coli is a single non-glycosylated polypeptide chain containing 72 amino acids. A fully biologically active molecule, rhIL-8 has a molecular mass of 8.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 8.4 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: SAKELRCQ CIKTYSKPFH PKFIKELRVI ESGPHCANTE IIVKLSDGRE LCLDPKENWV QRVVEKFLKR AENS
• Source: Escherichia coli.
• Biological Activity: ED50 5×10ˆ4 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human Interleukin-8°CXCL8 (rhIL-8) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-8 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: Interleukin-8, IL8, XCL8, monocyte-derived neutrop
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/mL.
• Expiration Date: 6 months from date of receipt.