Recombinant Human Nuclear Antigen Antibody

• Catalog Number: orb2635158
• Category: Antibodies
• Description: This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb235-1R recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 235-1R
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for the recombinant Human Nuclear Antigen antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-3ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Human Nuclear Antigen antibody is available for research use only.
• UniProt ID: Not Known
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Human Nuclear Antigen antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of MeOH-fixed human HeLa cells with recombinant Human Nuclear Antigen antibody (green, clone 235-1R).

Immunofluorescent staining of PFA-fixed human HeLa cells recombinant Human Nuclear Antigen antibody (green, clone 235-1R).

SDS-PAGE analysis of purified, BSA-free recombinant Human Nuclear Antigen antibody (clone 235-1R) as confirmation of integrity and purity.