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Catalog Number | orb1516425 |
---|---|
Category | Proteins |
Description | Recombinant human IL-18 protein |
Tag | Tag free |
Concentration | >0.5 mg/ml |
Form/Appearance | Liquid |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
MW | Due to glycosylation, the protein migrates to 19 kDa based on Tris-Bis PAGE result. |
Target | Interleukin-18 |
Protein Sequence | 37-193/193 |
Expression System | E.coli |
Biological Activity | Not tested |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
Alternative names | Interleukin-18; interferon gamma-inducing factor I Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
Recombinant Human IL-18 Protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Recombinant Human IL-18 Protein is greater than 95% as determined by SEC-HPLC.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 44.3 kDa after removal of the signal peptide.The apparent molecular mass of IL18-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Greater than 90% as determined by SDS-PAGE. | |
19.7 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |