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Catalog Number | orb2317438 |
---|---|
Category | Antibodies |
Description | Recombinant Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | rVU-1D9 |
Tested applications | FC, IHC |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Recombinant full-length human EpCAM protein |
Antibody Type | Recombinant Antibody |
Concentration | Purified Ab WITHOUT BSA and Azide at 1.0mg/ml |
Dilution range | Flow Cytometry (0.5-1ug/million cells); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined. |
Form/Appearance | 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Conjugation | Unconjugated |
MW | 40-43kDa |
Target | EPCAM |
Entrez | 4072 |
UniProt ID | P16422 |
Expression System | CHO cells |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Adenocarcinoma-associated Antigen; Cell Surface Gl Read more... |
Note | For research use only |
Application notes | Positive Control: MCF-7 or HT29 cells. Breast tumor |
Expiration Date | 12 months from date of receipt. |
FC, IHC | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC | |
Human, Mouse | |
Rabbit | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IHC | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |