Recombinant CD86 (Dendritic Cells Maturation Marker) Antibody

• Catalog Number: orb2317330
• Category: Antibodies
• Description: Recombinant CD86 (Dendritic Cells Maturation Marker) Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: C86/2160R
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human full-length CD86 protein
• Antibody Type: Recombinant Antibody
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 70kDa
• Target: CD86
• Entrez: 942
• UniProt ID: P42081
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Activation B7-2 antigen; B lymphocyte activation a
• Note: For research use only
• Application notes: Positive Control: 293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.
• Expiration Date: 12 months from date of receipt.