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Catalog Number | orb2310446 |
---|---|
Category | Antibodies |
Description | Recombinant CD54 / ICAM-1 Antibody |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | ICAM1/8247R |
Tested applications | IHC |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fragment raised against intracellular epitope of human ICAM1 (exact sequence is proprietary) |
Antibody Type | Recombinant Antibody |
Concentration | Purified Ab with BSA and Azide at 200ug/ml |
Dilution range | Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Conjugation | Unconjugated |
MW | 85-115kDa |
Target | ICAM1 |
Entrez | 3383 |
UniProt ID | P05362 |
Expression System | HEK293 cells |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | CD54; Cell surface glycoprotein P3.58; Human rhino Read more... |
Note | For research use only |
Application notes | Positive Control: Raji, HeLa or SW480 cells. Human tonsil. |
Expiration Date | 12 months from date of receipt. |