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Catalog Number | orb2317120 |
---|---|
Category | Antibodies |
Description | Recombinant CD45RB (B-Cell Marker) Antibody |
Target | PTPRC |
Clonality | Monoclonal |
Species/Host | Rabbit |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Concentration | Purified Ab with BSA and Azide at 200ug/ml |
Immunogen | Recombinant human full-length CD45RB protein |
UniProt ID | P08575 |
MW | 180-220kDa |
Tested applications | FC, IF, IHC, WB |
Dilution range | Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
Application notes | Positive Control: Ramos, U-698, or GA-10 cells. Human tonsil or spleen. |
Antibody Type | Recombinant Antibody |
Clone Number | PTPRC/1783R |
Expression System | CHO cells |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | B220, CD45R, GP180, Leukocyte common antigen (LCA) Read more... |
Note | For research use only |
Entrez | 5788 |
FC, IF, IHC-P, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |